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Nature of Parental Lentiviruses and Impact on Lentiviral Vector Development PowerPoint Presentation

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Nature of Parental Lentiviruses and Impact on Lentiviral Vector Development Presentation Transcript

Slide 1 - Nature of Parental Lentiviruses & Impact on Lentiviral Vector Development
Slide 2 - The human primate lentiviruses HIV-1 and HIV-2 are severe human pathogens, which target CD4+ T lymphocytes and macrophages. Other primates and non-primate lentiviruses are severe pathogens in their respective permissive hosts.
Slide 3 - DESIGN OF LENTIVIRAL VECTORS   It is evident that all possible means should be employed to reduce any pathogenicity associated with the wild-type lentivirus used as the basis for LV production and to minimise risks associated with LV.
Slide 4 - LENTIVIRAL VECTOR MANUFACTURING STRATEGIES LV are produced either by transient co-transfection of a permissive cell line with a combination of packaging and transfer vector constructs or by transfection and culture of suitable packaging cell lines.
Slide 5 - CHARACTERISATION AND CONTROL TESTING OF LENTIVIRAL VECTORS Any preparation of LV should be fully characterised with regard to transducing activity, other characteristics relevant to vector particles and the absence of RCL.
Slide 6 - LENTIVIRUS MANUFACTURING Lentivirus manufacturing solutions from Repligen can be explored using our interactive workflow exploring the different steps in this specific gene therapy. Different car gene vectors affect CART cell function, and currently used vectors are gamma retroviruses, lentiviruses, and CAR-T plasmid.
Slide 7 - Thank You Phone: +1 732 885 9188 / +1 877 436 7274 Email: cdmo.us@genscript.com Website: https://www.genscriptprobio.com/ Location: 860 Centennial Ave. Piscataway NJ 08854
 

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