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Dna Replication PowerPoint Presentation

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Dna Replication Presentation Transcript

Slide 1 - Nature of the Genetic Material Property 1 - it must contain, in a stable form, information encoding the organism’s structure, function, development and reproduction Property 2 - it must replicate accurately so progeny cells have the same genetic makeup Property 3 - it must be capable of some variation (mutation) to permit evolution
Slide 2 - Historical Perspective 1928 - Griffith discovered a “transforming principle” in heat killed bacteria (Property 1) 1944 - Avery demonstrated that the transforming principle is sensitive to DNase 1952 - Hershey & Chase used 32P and 35S labelling, of bacteriophage T2 DNA and protein respectively, to show only the DNA enters the host cell and can be passed to progeny phage (consistent with Property 2)
Slide 3 - Replication of DNA and Chromosomes Speed of DNA replication: 3,000 nucleotides/min in human 30,000 nucleotides/min in E.coli Accuracy of DNA replication: Very precise (1 error/1,000,000,000 nt)
Slide 4 - Meselson and Stahl (1958)
Slide 5 - ppt slide no 5 content not found
Slide 6 - Taylor and co-workers (1957) 3H-labelled chromosomes after one further replication in unlabelled media
Slide 7 - Bi-directional replication in E. coli
Slide 8 - Only One Replication Origin in E. coli
Slide 9 - Multiple Origins in Eukaryotes Eukaryotes replicate their DNA only in S-phase Eukaryotes have larger chromosomes Replication speed 2,600 npm. Largest Drosophila chromosome is 6.5 x 107 nucl., but it can replicate in 3-4 min. From a single origin, bidirectional replication would take 8.5 days. ==> The chromosome must have some 7,000 origins of replication.
Slide 10 - A replicating Drosophila chromosome
Slide 11 - Origins initiate replication at different times.
Slide 12 - Two DNA polymerases are involved in eukaryotic replication DNA polymerase d has no primase activity and is thought to be the polymerase that synthesizes the leading strand. DNA polymerase a has associated primase activity and is thought to be the polymerase that synthesizes the lagging strand.
Slide 13 - DNA Synthesis at the Origin Additional factors: PCNA (proliferating cell nuclear antigen) DNA helicase Replication factor C OTHERS
Slide 14 - Replication of Nucleosomes Eukaryotic DNA is packaged with histones in structures called nucleosomes. What happens to the nucleosome when the replication fork and the replication machinery pass by and open up the DNA double strand? Nucleosomes are found properly spaced on both postreplicative DNA strands immediately after passage of replication fork.
Slide 15 - ppt slide no 15 content not found
Slide 16 - A model for nucleosome replication
Slide 17 - The lagging strand of telomeres cannot be replicated by the usual mechanism
Slide 18 - Telomere and Telomerase Solution: special telomere sequence: tandem repeats of TTAGGG (human) telomerase, a specific enzyme with integrated RNA template.
Slide 19 - Telomere replication
Slide 20 - This powerpoint was kindly donated to www.worldofteaching.com http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.
 

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